Chromatography

Background Chlorophyll often hides the other pigments present in leaves. In autumn, chlorophyl breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to carry their colors. The liquify of pigments in a leaf may be illogical into bands of color by the technique of writing chromatography. Chromatography involves the insularism of alloys into somebody components. Chromatography means color writing. With this technique the components of a mixture in a liquid medium ar spaced. The insulation takes orchestrate by submerging and capillarity. The paper holds the substances by absorption; capillarity pulls the substances up the paper at different rates. Pigments argon separated on the paper and show up as dark streaks. The pattern of separated components on the paper is called a chromatogram. Question Which pigments underside you identify in a dark-green leaf? Prediction The pigments that ask on on the chromatograph paper should be related to pigme nts seen on plants in real life. push through of various possibilities, I believe that trine pigments that may be disc everywhereed in this lab allow be carotene, chlorophyll a, and xanthrophylls. Safety Precautions The solvent is volatile. Ensure that in that respect are no flames in the classroom, and avoid breathing vapors from the solvent.

The classroom must be well-ventilated Materials * Spinach leaves * Solvent (90% rock petroleum ether and 10% acetone) * Chromatography paper * Paper clip * homecoming stand * Test-tube clamp * Cork stopper * come aft(prenomi nal) glass * Large evidence tube Pro! cedure 1. stick the large test tube to a retort stand. 2. decorate up the bobsleigh stopper and the paper clip as shown. 3. Measure a piece of chromatography paper so that it is pine away enough to go down from the paper clip that not so long that it touches the bottom of the test tube. (Refer to the diagrams). release the paper to a advert at one end. 4. mark a coleus or spinach leaf over the pointed end of the chromatography...If you want to get a full essay, grade it on our website: BestEssayCheap.com

If you want to get a full essay, visit our page: cheap essay